Transmission of the EGFP Transgene in Zebrafish Progeny

Authors

  • Teodora Nikolovska Department of Genetics, Institute of Biology, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University-Skopje
  • Lozenka Ivanova Department of Genetics, Institute of Biology, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University-Skopje
  • Slobodan Tofiloski Saints Cyril and Methodius University in Skopje Faculty of Natural Sciences and Mathematics: Skopje, Skopje, MK
  • Gordana Dimeska Saints Cyril and Methodius University in Skopje Faculty of Natural Sciences and Mathematics: Skopje, Skopje, MK

DOI:

https://doi.org/10.59194/MJEE2527151n

Keywords:

Zebrafish, Genetics, Fluorescent, Transgene

Abstract

Zebrafish (Danio rerio) are widely used as model organisms in biomedical research, particularly in studies involving mutagenesis and transgenesis. Transgenic zebrafish, which express fluorescent proteins from a single transgene copy, are commercially available and exhibit distinct phenotypic traits. To investigate the inheritance pattern of the fluorescent phenotype, crossbreeding was performed between transgenic and wild-type individuals. The results revealed a marked difference in transgene inheritance depending on parental origin, with maternal transmission resulting in a significantly higher frequency of EGFP-positive offspring. Additionally, the appearance of wild-type individuals in the F1 generation from fluorescent parents suggests heterozygosity in the parental generation. These findings provide insights into the inheritance dynamics of the EGFP transgene and the genetic composition of the parental stock.

References

Bassett, D. & Currie, P.D., 2004. Identification of a zebrafish model of muscular dystrophy. Clinical and experimental pharmacology and physiology, 31: 537-540.

Gibbs, P.D.L. & Schmale, M.C., 2000. GFP as a genetic marker scorable throughout the life cycle of transgenic zebra fish. Marine Biotechnology, 2: 107-125.

Guo, S., 2004. Linking genes to brain, behavior and neurological diseases: what can we learn from zebrafish? Genes, Brain and Behavior, 3: 63-74.

Krøvel, A.V. & Olsen, L.C., 2002. Expression of a vas::EGFP transgene in primordial germ cells of the zebrafish. Mechanisms of development, 116: 141-150.

Linney, E., Hardison, N.L., Lonze, B.E., Lyons, S., DiNapoli, L., 1999. Transgene expression in zebrafish: a comparison of retroviral-vector and DNA-injection approaches. Developmental biology, 213: 207-216.

Mosimann, C. & Zon, L.I., 2011. Advanced zebrafish transgenesis with Tol2 and application for Cre/ lox recombination experiments. In Methods in cell biology (Vol. 104, pp. 173-194). Academic Press.

Patton, E.E. & Zon, L.I., 2005. Taking human cancer genes to the fish: a transgenic model of melanoma in zebrafish. Zebrafish, 1: 363-368.

Poss, K.D., Wilson, L.G., Keating, M.T., 2002. Heart regeneration in zebrafish. Science, 298: 2188-2190.

Poss, K.D., Keating, M.T., Nechiporuk, A., 2003. Tales of regeneration in zebrafish. Developmental dynamics: an official publication of the American Association of Anatomists, 226: 202-210.

Postlethwait, J., Amores, A., Force, A., Yan, Y.L., 1998. The zebrafish genome. Methods in cell biology, 60: 149-163.

Trede, N.S., Langenau, D.M., Traver, D., Look, A.T., Zon, L.I., 2004. The use of zebrafish to understand immunity. Immunity, 20: 367-379.

Udvadia, A.J. and Linney, E., 2003. Windows into development: historic, current, and future perspectives on transgenic zebrafish. Developmental Biology, 256(1), pp.1-17.

Vargas, R., Mackenzie, S., Rey, S., 2018. ‘Love at first sight’: the effect of personality and colouration patterns in the reproductive success of zebrafish (Danio rerio). PLoS One, 13: e0203320.

Vick, B.M., Pollak, A., Welsh, C., Liang, J.O., 2012. Learning the scientific method using GloFish. Zebrafish, 9: 226-241.

Westerfield, M., 2000. The zebrafish book: A guide for the laboratory use of zebrafish (Danio rerio). 4th ed. Univ. of Oregon Press, Eugene.

Downloads

Published

2025-07-04

How to Cite

Nikolovska, T. ., Ivanova, L., Tofiloski, S., & Dimeska, G. (2025). Transmission of the EGFP Transgene in Zebrafish Progeny. Macedonian Journal of Ecology and Environment, 27(1), 51–57. https://doi.org/10.59194/MJEE2527151n

Issue

Vol. 27 No. 1 (2025): Macedonian Journal of Ecology and Environment

Section

Articles

License

Copyright (c) 2025 Teodora Nikolovska, Lozenka Ivanova, Slobodan Tofiloski, Gordana Dimeska

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Macedonian Journal of Ecology and Environment applies the Creative Commons Attribution 4.0 International Creative Commons License license to articles and supplementary material we publish. If you submit your paper for publication to Macedonian Journal of Ecology and Environment, you agree to have the CC BY license applied to your work. Under this Open Access license, you as the author agree that anyone can reuse your article in whole or part for any purpose, for free, even for commercial purposes. Anyone may copy and redistribute the material in any medium or format as long as the author and original source are properly cited. This facilitates freedom in re-use and also ensures that Macedonian Journal of Ecology and Environment content can be mined without barriers for the needs of research.

Author - the holder of copyrights is encouraged to sign and return the Copyright form prior to the publication of the scientific article.

Licencing:

Creative Commons License
Macedonian Journal of Ecology and Environment is licensed under a Creative Commons Attribution 4.0 International License.