Breaking Seed Dormancy in Pili (Canarium ovatum): Efficacy of Hot Water, Cold Water, and Scarification Treatments
Keywords:
percentage germination, percentage germinative energy, percentage germinative capacity, percent survival, pre-germination treatmentsAbstract
Pili (Canarium ovatum) is an indigenous tree species in the Philippines valued for its edible nuts, oil, and economic importance. However, propagation of Pili is often limited by seed dormancy, which delays germination and reduces seedling production efficiency. Limited information is available regarding effective pre-germination treatments that can improve the germination performance of Pili seeds. Hence, this study aimed to evaluate the effects of different pre-germination treatments on the germination performance of Pili seeds. Specifically, the study assessed percent survival, percentage germination, germinative energy, and germinative capacity under four treatments: control (untreated), hot water soaking, cold water soaking, and scarification. A total of 320 seeds were arranged in a Randomized Complete Block Design (RCBD) with four treatments replicated four times. Hot water soaking involved immersing seeds in 100°C water for 15 seconds, cold water soaking involved soaking seeds in 10–20°C water for 12 hours, while scarification was performed through manual nicking using secateurs.
Results showed that hot water soaking produced the highest seed survival, germination percentage, germinative energy, and germinative capacity among all treatments tested. Analysis of variance (ANOVA) revealed highly significant differences among treatments for percent survival (F = 804.60, p < 0.0000), percentage germination (F = 3092.74, p < 0.0000), and germinative capacity (F = 9720.18, p < 0.0000). The findings indicate that hot water soaking is the most effective pre-germination treatment for breaking seed dormancy and improving the germination performance of Pili seeds.
References
Abere, M., Eshete, A., & Alemu, A. (2022). Traditional uses and local management practices of Adansonia digitata L. in different ethnic groups of Quara wereda, North West low land of Ethiopia. Trees, Forests and People, 7, 100188.
Amoakoh, O. A., Nortey, D. D. N., Sagoe, F., Amoako, P. K., & Jallah, C. K. (2017). Effects of pre-sowing treatments on the germination and early growth performance of Pouteria campachiana. Forest science and technology, 13(2), 83- 86.
Anwar, R., & Malik, A. U. (2007). Hot water treatment affects ripening quality and storage life of mango (Mangifera indica L.). Pakistan Journal of Agricultural Sciences, 44(2), 304- 311.
Anozie, E. L., & Oboho, E. G. (2019). The effect of seed source and pre-sowing treatment on germination of Canarium schweinfurthii [Engl] seeds. Asian J. Res. Agric. For, 4, 1-11.
Bhardwaj R. L. (2014). Effect of growing media on seed ger¬mination and seedling growth of papaya cv. ‘Red lady’. African Journal of Plant Science, 8(4): 178-184. https:// academicjournals.org/article/article1398438500.
Coronel, R. E. (1996). Pili nut, Canarium ovatum Engl (Vol. 6). Bioversity International.
Dai, L., Chen, Y., & Wei, X. (2023). Hard seeds characteristics and seed vigor of Ormosia hosiei. Agriculture, 13(5), 1077.
Fabiao, A., Faria, C., Almeida, M. H., & Fabião, A. (2014). Influence of mother plant and scarification agents on seed germination rate and vigor in Retama sphaerocarpa L.(Boissier). Forest-Biogeosciences and Forestry, 7(5), 306.
Fernandes T. V., Paolucci, L. N., Carmo, F. M. S., Sperber, C. F., & Campos, R. I. (2018). Seed manipulation by ants: dis¬entangling the effects of ant behaviours on seed germination. Ecological Entomology, 43(6): 712-718. https://doi. org/10.1111/een.12655.
Fredrick, C., Muthuri, C., Ngamau, K., & Sinclair, F. (2017). Provenance and pretreatment effect on seed germination of six provenances of Faidherbia albida (Delile) A. Chev. Agroforestry System, 91: 1007-1017.
Guzzon, F., Costich, D. E., Afzal, I., Barquero, L. B., Vargas, M. A. A., Ramirez, E. V., Bello, P., Dahal, P., Cano, C. S., Espinosa, C. Z., Imran, S., Patolo, S., Tukia, T. N., Asbrouk, J. V., Nabubuniyaka-Young, E., Gianella, M., & Bradford, K. J. (2024). Applications of dry chain technology to maintain high seed viability in tropical climates. Peer Journal. Retrieved on 28 May 2025 from https://doi.107717/ peerj.18146.
Herter, U., & Burris, J. S. (1989). Effect of drying rate and temperature on drying injury of corn seed. Canadian Journal of Plant Science, 69(3), 763-774.
Khalil, K., &Suleman, Y. (2019). Scarification in Acetic Acid, Hot Water, and Cold Sand Stratification to Improve Carob (Ceratonia siliqua L.) Seed Germination. Tikrit Journal for Agricultural Sciences, 19(1), 118-125.
Mendioro, M. S., Diaz, M. G. Q., Velasco, V. M. E., Alcaraz, M. C., Lalamunan, R. C., Amoloza, K. G., & Villamael, L. N. (2008). Genetic characterization of pili (Canarium ovatum Engl.) from Albay, Camarines Norte, and Camarines Sur through isozyme analysis. Philippine Journal of Science, 137(2), 115-125.
Mewded, B., Lemessa, D., Negussie, H., & Berhanu, A. (2019). Germination pretreatment and storage behavior of Terminalia laxiflora seed. Journal of Forestry Research, 30(4): 1337-1342.
Mganga, M., & Mziho, P. (2025). The potential of selected seed pretreatment methods to initiate and speed up germination. Journal of Science and Technology, 43(1): 147-163. https://webapps.knust.edu.gh/just/index.
Millena, C. G., & Sagum, R. S. (2018). Physicochemical characterization and fatty acid profiling of different Philippine pili nut (Canarium ovatum, Engl.) varieties. Journal of the American Oil Chemists’ Society, 95(3), 325-336.
Missanjo, E., Chioza, A., & Kulapani, C. (2014). Effects of Different Pretreatments to the Seed of Seedling Emergence and Growth of Acacia polyacantha. International Journal of Forestry Research, 2014: 6. https://doi. org/10.1155/2014/583069
Nurdin, A. S., Hadun, R., Setiawan, B. D., Irmayanti, L., & Ashari, R. (2022). Effects of Initial Water Temperature and Soaking Duration on the Germination of Canarium (Canarium indicum) Seeds. Jurnal Sylva Lestari, 10(2), 244-253.
Obiazi, C. C. (2015). Hot water enhanced germination of Leucaena leucocephala seeds in light and dark conditions. Current Research in Agricultural Sciences, 2(2), 67-72.
Obideen, R., Alhasan, D. A., & Draie, R. (2024). Effect of Some Treatments on Seed and Vegetative Propagation of Haw¬thorn (Crataegus spp.) in Northwestern Syria. International Research Journal of Innovations in Engineering and Technology, 8(10): 39-46. https://doi.org/10.47001/IRJI¬ET/2024.810007.
Odoi, J. A., Osei, J. K., & Osei, P. (2020). Effect of soaking treatments on germination of hard-coated tropical forest tree seeds. Uganda Journal of Agricultural Sciences, 19(2): 5-10. https://www.ajol.info/index.php/ujas/article/view/204767.
Ogbu Justin, U., & Otah Okocha, I. (2017). Germination response of velvet tamarind (Dialium guineense Willd.) seeds treated with pre-sowing soaking in water at varying temperatures and durations.
Olujobi, O., Thompson, Z. O., & Faniseyi, A. S. (2022). Effect of acid pre-treatment and potting media on seed germination and early seedling growth of Albizia lebbek. Journal of Applied and Natural Science, 16(3): 123-130.https://doi. org/10.30574/gscbps.2022.20.3.0338.
Pabalan, C., & Aquino, A. J. (2020). Germination performance of Mangkono (Xanthostemon verdugonianus). Asian Journal of Research in Agriculture and Forestry, 6(4): 58-71. https://www.researchgate.net/publication/362815360.
Paguit, J. C., Luceño-Tenizo, A. J. M., & Coritico, F. P. (2024). Effects of the Hot Water Pre-treatments and Storage Durations on the Seed Germination of Falcata (Falcataria falcata (L.) Greuter & R. Rankin). Bangladesh Journal of Botany, 53(2), 249-255.
Seid, M. A., Wondimu, T., Degu, A., & Assefa, A. (2023). Seed Germination Enhancement of Two Balanites Species (B. aegyptiaca (L.) Del. and B. rotundifolia (Tiegh.) Blatt.) Using Different Presowing Treatments in Ethiopia. Scientifica, 2023(1), 5571489.
Spadoni, A., Guidarelli, M., Sanzani, S. M., Ippolito, A., & Mari, M. (2014). Influence of hot water treatment on brown rot of peach and rapid fruit response to heat stress. Post-harvest Biology and Technology, 94, 66-73.
Tacloy, J. G., Bao-Idang, C, C., Ngiwas, S, L., Esteban, M. B., & Yabes, M. D. (2022). Domestication of “Denguai” (Saurauia bontocensis Merr.) at La Trinidad, Benguet, Philippines. Philippine Journal of Sciences, 151(1), 157-169.
Tahing, A., Semang, A., & Vertygo, S. (2024). The Effect of Hot Water Scarification Duration on Germination and Growth of Indigofera zollingeriana Seeds. Journal Biologi Tropis, 24(2): 318-324. https://doi.org/10.29303/jbt.v24i2.6848
Uttam Thangjam, U. T., & Sahoo, U. K. (2017). Effects of differ¬ent pre-treatments and germination media on seed germination and seedling growth of Parkia timoriana (DC.) Merr.
Vicient, C. (2017). The effect of frequency-specific sound signals on the germination of maize seeds. BioMed Central Research Notes, 10: 323. https://doi.org/10.1186/s13104- 017-2643-4.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Sheryl M. Ducusin, Christine Joy C. Yanguas, Rea May P. Talicuad, Paul U. Catusalem, Ashlie A. Tagle, Jan Orville P. Bautista, Novelyn D. Buhong

This work is licensed under a Creative Commons Attribution 4.0 International License.
Macedonian Journal of Ecology and Environment applies the Creative Commons Attribution 4.0 International
license to articles and supplementary material we publish. If you submit your paper for publication to Macedonian Journal of Ecology and Environment, you agree to have the CC BY license applied to your work. Under this Open Access license, you as the author agree that anyone can reuse your article in whole or part for any purpose, for free, even for commercial purposes. Anyone may copy and redistribute the material in any medium or format as long as the author and original source are properly cited. This facilitates freedom in re-use and also ensures that Macedonian Journal of Ecology and Environment content can be mined without barriers for the needs of research.
Author - the holder of copyrights is encouraged to sign and return the Copyright form prior to the publication of the scientific article.
Licencing:

Macedonian Journal of Ecology and Environment is licensed under a Creative Commons Attribution 4.0 International License.
